Corrigendum to “Bombesin-like receptor 3 (Brs3) expression in glutamatergic, but not GABAergic, neurons is required for regulation of energy metabolism” [Mol Metabol 6 (2017) 1540–1550]

نویسندگان

  • Cuiying Xiao
  • Ramón A. Piñol
  • Jesse Lea Carlin
  • Cuiling Li
  • Chuxia Deng
  • Oksana Gavrilova
  • Marc L. Reitman
چکیده

In the original article, the PCR genotyping protocol contained errors. The genotyping used primers x213 (50-GTATGCAT TACCACGTACGA, intron correct x214 primer sequence is 50-TTTCTCTGGGAATGACAATGC. The correct PCR product sizes are 191 bp for the wild type allele and 249 bp for the floxed allele. These corrections do not affect the conclusions of the paper, and the authors sincerely apologize for this oversight. Generation of Brs3 conditional allele, Brs3 having the floxed targeted allele with the selection cassette removed was selected. PCR

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منابع مشابه

Bombesin-like receptor 3 (Brs3) expression in glutamatergic, but not GABAergic, neurons is required for regulation of energy metabolism

OBJECTIVE Bombesin-like receptor 3 (BRS-3) is an orphan G protein-coupled receptor. Brs3 null mice have reduced resting metabolic rate and body temperature, increased food intake, and obesity. Here we study the role of Brs3 in different neuron types. METHODS Mice able to undergo Cre recombinase-dependent inactivation or re-expression of Brs3 were generated, respectively Brs3fl/y and Brs3loxTB...

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Regulation of body temperature and brown adipose tissue thermogenesis by bombesin receptor subtype-3.

Bombesin receptor subtype-3 (BRS-3) regulates energy homeostasis, with Brs3 knockout (Brs3(-/y)) mice being hypometabolic, hypothermic, and hyperphagic and developing obesity. We now report that the reduced body temperature is more readily detected if body temperature is analyzed as a function of physical activity level and light/dark phase. Physical activity level correlated best with body tem...

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Search for an Endogenous Bombesin-Like Receptor 3 (BRS-3) Ligand Using Parabiotic Mice

Bombesin-like receptor 3 (BRS-3) is an X-linked G protein-coupled receptor involved in the regulation of energy homeostasis. Brs3 null (Brs3-/y) mice become obese. To date, no high affinity endogenous ligand has been identified. In an effort to detect a circulating endogenous BRS-3 ligand, we generated parabiotic pairs of mice between Brs3-/y and wild type (WT) mice or between WT controls. Succ...

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Bombesin-like receptor 3 regulates blood pressure and heart rate via a central sympathetic mechanism.

Bombesin-like receptor 3 (BRS-3) is an orphan G protein-coupled receptor that regulates energy expenditure, food intake, and body weight. We examined the effects of BRS-3 deletion and activation on blood pressure and heart rate. In free-living, telemetered Brs3 null mice the resting heart rate was 10% lower than wild-type controls, while the resting mean arterial pressure was unchanged. During ...

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Glutamatergic drive facilitates synaptic inhibition of dorsal vagal motor neurons after experimentally induced diabetes in mice.

The role of central regulatory circuits in modulating diabetes-associated glucose dysregulation has only recently been under rigorous investigation. One brain region of interest is the dorsal motor nucleus of the vagus (DMV), which contains preganglionic parasympathetic motor neurons that regulate subdiaphragmatic visceral function. Previous research has demonstrated that glutamatergic and GABA...

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عنوان ژورنال:

دوره 9  شماره 

صفحات  -

تاریخ انتشار 2018